The Roos had fought tooth and nail to don their much-loved royal blue and white striped home guernsey against the Magpies, but barely uttered a yelp on their way to a demoralising 87-point defeat.
"The players have had a reality check today," Scott said.
"This was about turning up and wearing our home jumper in a home game, which is our right. I was more than happy to put it on our players and put them under pressure today and really ask them to stand up and be counted against a genuinely good opposition.
"It was really an opportunity for our club to stand up and be counted and on the football front, from coaches to players, we failed dismally. It's a bitterly disappointing performance.
"We fell short, but I'll never stop doing that. We've got to demand excellence against the best. We're not there yet, but we'll get there or we'll die trying."
The blowout continues a trend of disappointing performances against top-four quality sides and Scott agreed his players simply don't yet believe they can match it with that calibre of opposition.
Scott didn't absolve himself of blame for the heavy loss that was orchestrated by virtuoso performances from Dane Swan and Scott Pendlebury.
"We chose to play Collingwood a certain way because we wanted to take them on at their own game," he said.
"There's no point going down in an honourable loss - I don't think it helps us in the long term. We want to take Dane Swan on in the midfield, we want to take Scott Pendlebury on, but we fell well, well short and that was obvious to all.
"Their pressure is outstanding and we couldn't stand up to that pressure. I was hoping Collingwood's pressure would be that good because we wanted to get a measure of where we're at and unfortunately, it's a pretty ordinary measure."
Brent Harvey and Brady Rawlings were among the few Kangaroos' to at least break even on the day. The pair topped the team's possession count, but had precious little support from their younger teammates around the ball.
"We're not going to get to where we want to go if we're continually relying on the same guys every week," he said.
"We're trying to shed this image of a young, developing football team and we did nothing to shed that image today.
"We need the younger guys to step up. They've got to grow up, and grow up quickly. They are genuine competitors so this will sting them and this will hurt them, but it's going to mean nothing unless we go back and rectify it in two weeks time against Fremantle."
